Reversible Nuclear-Lipid-Droplet Morphology Induced by Oleic Acid: A Link to Cellular-Lipid Metabolism
Fig 3
Analysis of cLD and nLD populations from rat primary hepatocytes by fluorescence microscopy.
Nuclei (N) and lipid droplets (nLD and cLD) were stained with DAPI (blue) and BODIPY 493/503 (green), respectively. The red and yellow arrows indicate the cLD and nLD, respectively. Control, cells cultured under control conditions for 24 h; 400 OA, cells treated with 400 μM OA for 24 h; 400 OA + 5TC: cells treated with 400 μM OA plus 5 μM TC for 24 h;–OA (48) and–OA (72), cells treated with 400 μM OA for 24h and then incubated in the absence of OA for 48 or 72 h, respectively. The photographs correspond to representative observations.