Cudraflavone C Induces Tumor-Specific Apoptosis in Colorectal Cancer Cells through Inhibition of the Phosphoinositide 3-Kinase (PI3K)-AKT Pathway
Fig 5
Ectopic expression of myr-AKT confers resistance to cudraflavone C.
(A) Caco2 and KM12 cells were transfected with myristoylated AKT for 24 hours before treatment of cells with test agent. Lysates were collected at 48 hours after transfection for immunoblotting analysis. (B) Cell proliferation was quantified by CellTiterGlo® and (C) Apoptotic cell death was quantified by annexin V/7-AAD flow cytometry. All data represent the mean ± s.d. from at least three independent experiments. Symbol “*” presents the statistical significance concluded from Student’s independent t-test with p-value ≤0.05.