Validation of Synthetic CRISPR Reagents as a Tool for Arrayed Functional Genomic Screening
Fig 5
Evaluation of screen results from synthetic CRISPR and siRNA screens of ubiquitin system genes.
(A) RSA reveals many more significant hits (FDR < 0.05) for CRISPR relative to siRNA screening. (B) Genes identified through CRISPR screening with a RSA corrected p-values < 0.001 and corresponding values from siRNA screening. “OTP siRNA” refers to results from screening of the Dharmacon On-Target Plus siRNA library. “SilSelect siRNA” refers to results from screening the Ambion Silencer Select siRNA library. “All siRNA” refers to results obtained after combing data from both siRNA screens. *Refers to crRNA screen hits that appear to be supported by siRNA data. (C) Genes with RSA corrected p-value < 0.001 in the CRISPR screen are associated with progression through the cell cycle.