Mapping of Complete Set of Ribose and Base Modifications of Yeast rRNA by RP-HPLC and Mung Bean Nuclease Assay
Fig 5
Identification and mapping of complete set of ribose and base modifications of 25S rRNA.
Composition analysis of the 68 discrete fragments isolated from 25S rRNA using MBN protection assay. Overlaid RP-HPLC chromatograms of different fragments identified to contain (A) Am residues; (B) Gm residues; (C) Cm residues; (D) Um residues; (E) m1A; (F) m5C; and (G) m3U residues. Location of each modification along with the oligonucleotide number used for the isolation of respective fragment are stated on the right side of the peak. For the fragments containing more than one modifications, e.g. oligo 45, oligo 46 for Am, oligo 18 and 56 for Gm, and oligo 49 and 55 for Um, strategy explained above in Fig 2C were used to map them to exact position.