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Adenosine Stimulate Proliferation and Migration in Triple Negative Breast Cancer Cells

Fig 5

The adenylate cyclase/PKA pathway is involved in the effects of Ado in MDA-MB-231 cells.

Incubation of MDA-MB-231 cells in the presence of an adenylate cyclase agonist (FSK; 10 μM) mimics the Ado-induced effect on cell proliferation (A) and migration (B). In contrast, incubation in the presence of the antagonist KT-5720 (500 nM) prevented the effect of Ado on MDA-MB-231 cell proliferation and migration. (C) The activation of Ado receptors in MDA-MB-231 cells causes a concentration-dependent increase of [3H]-cAMP.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0167445.g005