Silver Nanoparticle-Directed Mast Cell Degranulation Is Mediated through Calcium and PI3K Signaling Independent of the High Affinity IgE Receptor
Fig 5
Involvement of other signaling pathways in response to AgNPs
Mast cell degranulation was assessed by measuring the release of β-hexosaminidase into supernatants. Cells were pre-treated with (A) the sphingosine kinase inhibitor DMS (1 μM) or (B) the PKC inhibitor Ro31-8220 (10 μM) for 30 min prior to AgNP (25 μg/ml) exposure for 1 h and release of β-hexosaminidase was assessed. Values are expressed as mean ± SEM of at least 3 independent experiments. *Indicates significant difference from controlled group (p≤0.05). #Indicates significant difference from AgNP-treated group (p≤0.05)