Silver Nanoparticle-Directed Mast Cell Degranulation Is Mediated through Calcium and PI3K Signaling Independent of the High Affinity IgE Receptor
Fig 2
Mast cell degranulation following exposure to AgNPs
Mast cell degranulation was assessed by measuring the release of β-hexosaminidase into supernatants. (A) Cells were pre-treated with the SR-B1 inhibitor Blt-2 (50 μM), SR-B1 specific antibodies (1:100 dilution) 30 min prior to AgNP (25 μg/ml) exposure for 1h and release of β-hexosaminidase was assessed. (B) Cells were sensitized with anti-DNP IgE overnight and then exposed to AgNPs (25 μg/ml) for 1 h and release of β-hexosaminidase was assessed. (C) Cells were pre-treated with Blt-2 (50 μM) for 30 min then activated with either DNP (30 min) or AgNP (1 h) and release of β-hexosaminidase was assessed. (D) Representative immunoblots for global p-Tyr and p-Ser/Thr following DNP (100 ng/ml) or AgNPs (25 μg/ml) exposure. Values are expressed as mean ± SEM of at least 3 independent experiments. *Indicates significant difference from controlled group (p≤0.05). #Indicates significant difference from AgNP-treated group (p≤0.05)