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Exendin-4 Inhibits Hepatic Lipogenesis by Increasing β-Catenin Signaling

Fig 1

Palmitic acid stimulates lipid accumulation in hepatocytes.

(A) HepG2 cells were treated with 200–500 μM palmitic acid (PA) for 24 hours. Triglycerides were extracted from cultured cells and quantitated by enzymatic assays. Triglyceride levels were normalized to total cellular protein contents. (B) HepG2 cells were treated with 400 μM PA for 24 hours. The mRNA expression levels of perilipin 1 (PLIN1), perilipin 2 (PLIN2) and perilipin 3 (PLIN3) were normalized to the level of β-actin. All values are expressed as the mean ± SE (n = 6). * p<0.05, ** p<0.01 compared with control cells.

Fig 1

doi: https://doi.org/10.1371/journal.pone.0166913.g001