Cyclic Compressive Stress Regulates Apoptosis in Rat Osteoblasts: Involvement of PI3K/Akt and JNK MAPK Signaling Pathways
Fig 5
Effect of large-magnitude mechanical stress-induced cell apoptosis in osteoblasts pretreated with or without specific inhibitors.
Osteoblasts were pretreated with or without specific inhibitors for 1 h and then treated with mechanical stress of 0.6Hz×8cm (0 as control) for 30min and then cultured for a further 24h. (A) Apoptosis assayed by flow cytometry using annexin-V/PI double staining. (B) Quantitative analysis of apoptotic osteoblasts in each group. *P<0.05 versus control group; #P<0.05 versus loaded group. Data are presented as mean ± SD of three independent experiments.