Tumor Necrosis Factor-Alpha and the ERK Pathway Drive Chemerin Expression in Response to Hypoxia in Cultured Human Coronary Artery Endothelial Cells
Fig 5
Hypoxia increases proliferation of HCAECs.
Incorporation of [3H]thymidine into HCAECs increased during 2.5% O2 hypoxia and after addition of exogenous chemerin or TNF-alpha during normoxia for 2 to 4 h. Hypoxia-induced incorporation of [3H]thymidine into HCAECs was suppressed by chemerin siRNA, PD98059, or by the anti-TNF-alpha antibody (Ab; n = 4 per group); *p < 0.01 compared to control, §p < 0.01 compared to hypoxia. Cpm: counts per minute.