Deep Brain Photoreceptor (val-opsin) Gene Knockout Using CRISPR/Cas Affects Chorion Formation and Embryonic Hatching in the Zebrafish
Fig 5
Late-hatching embryos obtained from a F0 male valopa mutant.
(a) Images of the zebrafish embryos obtained from a male WT sibling and a male mutant valoparw03 (represented by A3) at 0 day-post-fertilization (dpf), 4 dpf, and 6 dpf. (b and c) the mortality rates of the eggs or embryos and hatching rates of the embryos, respectively. Most embryos from the mutant had not hatched at 4 dpf, in contrast to most embryos from the WT sibling hatched. At 6 dpf, most of the unhatched embryos had died in the chorion. Genotyping of F1 adult offspring identified a mixture of WT and mutants. Note that the association between the valop mutations in individual F0 parents and the decreased number of hatched embryos at 4 dpf was statistically significant (determined by Chi-Square X2 values and p-values). Logistic Regression analysis showed that the observed phenotype is likely dependent on the targeted mutation (data in S1 File). N represents the number of eggs or embryos; and percentages shown in a single bar add up to a total of 100%. Scale bar, 1 mm.