Inositol Pyrophosphate Profiling of Two HCT116 Cell Lines Uncovers Variation in InsP8 Levels

doi:10.1371/journal.pone.0165286

Inositol Pyrophosphate Profiling of Two HCT116 Cell Lines Uncovers Variation in InsP₈ Levels

Fig 4

Separation of 1-InsP₇ and 5-InsP₇ by CarboPac HPLC.

Standards of [³H]InsP₆, 1-[³H]InsP₇, 5-[³H]InsP₇, and [³H]InsP₈ (1 nmol of each) were chromatographed on a CarboPac HPLC column. Panels A and B show HPLC runs in which either 1-[³H]InsP₇ or 5-[³H]InsP₇ were added individually, while Panel C shows an HPLC run in which both [³H]InsP₇ isomers were added together. Panel D, the mass amount of 5-InsP₇ was increased to 20 nmol.

doi: https://doi.org/10.1371/journal.pone.0165286.g004