Implementation of Novel Design Features for qPCR-Based eDNA Assessment
Fig 5
Determination of the suitability of environmental samples for eDNA qPCR assessment–Part 1 of a tripartite eDNA assay.
The ePlant5 primer set was used to detect a 147 base pair region of the chloroplast 23S ribosomal RNA gene. Samples were assessed in duplicate and included 5 different environmental water sources (A), municipal tap water (B), human total DNA (C), and distilled water (D). The dashed vertical line denotes the selected data collection cut-off for use in scoring samples based upon the presence of an amplifiable environmental DNA source.