Targeting Epithelial-Mesenchymal Transition for Identification of Inhibitors for Pancreatic Cancer Cell Invasion and Tumor Spheres Formation
Fig 10
Inhibition of pancreato-spheres formation by BSI analogues.
BxPC-3 and PANC-1 cells were seeded in ultra-low attachment plates and then cells were exposed to 25 μM of BSI analogues. BxPC-3 (A) and PANC-1 (F) primary spheres were imaged and counted 14 days post treatment. Bar graph representing the average number of primary spheres of BxPC-3 (B) and PANC-1 (G), feret diameter of the primary spheres of BxPC-3 (C) and PANC-1 (H) spheres± SEM. Primary spheres were dissociated into individual cells using trypsin and then reseeded into ultra-low attachment plates for secondary spheres. Again cells were exposed to 25 μM of BSI analogues. BxPC-3 (A) and PANC-1 (F) Secondary Spheres were imaged and counted 14 days post treatment. Bar graphs representing the average number of secondary spheres ± SD of BxPC-3 (D) and PANC-1 (I) cells, feret diameter of BxPC-3 (E) and PANC-1 (J) spheres. Scale bar 500 μm. Magnification of the images 100X.