A Novel Class of Small Molecule Agonists with Preference for Human over Mouse TLR4 Activation
Fig 10
Ugi compounds activate mouse BMDCs that express human TLR4/MD2.
Bone marrow cells were isolated from femur/tibia bones of w.t. C57BL/6, TLR4/MD2 KO, and hTLR4/MD2 KIKO mice and cultured with GM-CSF. BMDCs were harvested at day 9, washed, counted, and stimulated in vitro for 24 h with 1 μg/ml TLR4 agonists and 2 μM R848. SNs were analyzed via mouse MILLIPLEX kits. Data are expressed as the means of 2 mice and are representative of 2 studies.