A Novel Class of Small Molecule Agonists with Preference for Human over Mouse TLR4 Activation
Fig 6
The Ugi compound-induced cytokine profile is similar to MPL and LPS in human PBMCs but not mouse splenocytes.
PBMCs were isolated from three healthy volunteers, while murine splenocytes were isolated from three naïve BALB/c mice. TLR4 agonists were used at 1 and 0.2 μg/ml doses to stimulate human PBMCs and murine splenocytes for 24 hrs. SNs were analyzed via human and mouse MILLIPLEX kits. Data are expressed as the means of 3 donors/mice at the 1 μg/ml dose of TLR4 agonist and are representative of 2 studies. Raw data are presented in S3 Fig.