Functional Properties of Mouse Chitotriosidase Expressed in the Periplasmic Space of Escherichia coli
Fig 5
Degradation of colloidal and crystalline chitin and various GlcNAc oligomers by the recombinant Chit1.
Crystalline chitin (A), colloidal chitin (B), (GlcNAc)6 (C), (GlcNAc)5 (D), (GlcNAc)4 (E) and (GlcNAc)3 (F) were used as substrates in McIlvaine's buffer (pH 5.0). Reactions were conducted for 10 min, 1 h or 16 h at 37°C. Chitin fragments generated by the recombinant proteins were analyzed by fluorophore-assisted carbohydrate electrophoresis. Chitin oligomers are shown in the left margin as standards. Protein A-Chit1-V5-His released primarily (GlcNAc)2 fragments from chitin substrates.