Generating Rho-0 Cells Using Mesenchymal Stem Cell Lines
Fig 7
mRNA expression of mitochondrial biogenesis genes in 3a6 without treatment (3a6-wt) and 3a6 Rho-0 cells.
(a). To analyze mitochondrial biogenesis, peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) and transcription factor A, mitochondrial (TFAM) gene expression levels were determined under basal conditions. (b). Fold increase of PGC-1α gene levels during osteogenic and adipogenic induction. and (c). TFAM gene levels during osteogenic and adipogenic induction. Data are expressed relative to basal condition (basal condition equal to 1). All data were obtained from two independent experiments, expressed as mean ± SD and analyzed by the unpaired t-test (* p<0.05; ** p<0.005). Basal, in standard medium, Adipo (Adipogenic) and Osteo (Osteogenic); all experiments were developed during 9 days in each specific differentiation culture medium.