Generating Rho-0 Cells Using Mesenchymal Stem Cell Lines
Fig 4
The response of the KP cell line to different substances that deplete mitochondrial DNA.
All mtDNA copy numbers are expressed as percentages compared to those of untreated cells valued at 100%. (a). Treatment with ethidium bromide (EtBr) at three different concentrations, 1 mM, 500 nM and 100 nM, for 240 hours. (b). Treatment with Rhodamine6g (Rd6g) at 3 μg/ml for 24, 48, 72 and 96 hours; the positive response started at 48 hours. (c). Treatment with Zidovudine (AZT) at 10 μM and 0.5 mM for 168 and 240 hours. (d). Treatment with Stavudine (d4t) at three concentrations, 5 μM, 10 μM and 0.5 mM, for 240 hours. 8x104 cells were plated in each experiment for treatment with different agents. The figures represent at least two independent experiments.