Generating Rho-0 Cells Using Mesenchymal Stem Cell Lines
Fig 3
(a). The mitochondrial DNA (mtDNA) copy number of 15 clones isolated by limit dilution from 3a6 cells treated with Stavudine (d4t) at 0.5 mM for 240 hours. Data for clones are expressed as percentages of untreated cells [without treatment (wt), value 100%]. (b). mtDNA copy number of two selected clones, one with low mtDNA (C-3; 1.57%) and the other with a high value for this parameter (C-10; 0.80%). Both clones were re-treated with d4t at 0.5 mM for 192 hours with mtDNA copy number expressed as a % of that of the 3a6 parental line. In this graphic, the %mtDNA copy number for 143B.TK- Rho-0 is also represented in relation to that of its parental cell line (143B.TK-). (c). This graph shows the % of mtDNA copy number for C-3 cultured in DMEM without d4t for 288 hours. The results show the stability of the mtDNA copy number during culture without the drug.