Signaling Properties of Chemerin Receptors CMKLR1, GPR1 and CCRL2
Fig 10
Contribution of Gi/o proteins and arrestins to CMKLR1- and GPR1-mediated ERK1/2 phosphorylation.
Mouse embryonic fibroblasts (MEF) expressing CMKLR1 or GPR1 were stimulated with 300 nM chemerin (wlack bars) or buffer only (white bars) for two minutes and phosphorylation of ERK1/2 estimated by Western blotting. Results are expressed as the ratio between the amounts of phospho-ERK1/2 and total ERK1/2 following quantification. Data represent the mean ± S.E.M. of three independent experiments. MEF cells were derived form β-Arr1 KO and WT1 or β-Arr2 KO and WT2 siblings. Data represent the mean ± S.E.M. of three independent experiments. Statistical significance was assessed using Tukey's test (***P < 0.0001; ** and ## P< 0.001; * and # P< 0.01).