Signaling Properties of Chemerin Receptors CMKLR1, GPR1 and CCRL2
Fig 9
Functional response of chemerin receptors.
A. Calcium mobilization was measured in CHO-K1 cells using the aequorin-based functional assay. Cells expressing chemerin receptors were stimulated with increasing concentrations of chemerin and luminescence was recorded for 30 s. The results were normalized for the basal luminescence of the cells in absence of agonist (0%) and the maximal response obtained for each cell line with 10 μM ATP (100%). B. Enlarged panel derived from Fig 9 A showing that GPR1 signal triggered by 3 μM chemerin accounts for about 15% of the CMKLR1 signal. Data represent the mean ± S.E.M. of three independent experiments C. Immunoblot detection of phosphorylated ERK1/2 MAP kinases revealed with anti-phospho ERK1/2 (upper panel). CHO-K1 cells expressing chemerin receptors were stimulated with 300 nM chemerin for various times. Detection of total ERK1/2 by Western blotting was used to ascertain that an equal amount of material was loaded in each lane (lower panel). A typical experiment out of three performed independently is shown.