Signaling Properties of Chemerin Receptors CMKLR1, GPR1 and CCRL2
Fig 7
Impact of Pertussis toxin on arrestins recruitment to CMKLR1 and GPR1.
Real-time measurement of BRET signal in HEK293T cells expressing β-arrestin1-Rluc (A-B) or β-arrestin2-Rluc (C-D) in combination with CMKLR1-Venus or GPR1-Venus, following stimulation with 100 nM chemerin in the absence (●) or the presence of Pertussis toxin (PTX, ◯). Results of BRET experiments are expressed as the difference in BRET signals measured in the presence and absence of chemerin. Data represent the mean ± S.E.M. of three independent experiments.