Signaling Properties of Chemerin Receptors CMKLR1, GPR1 and CCRL2
Fig 6
Recruitment of β-arrestins by CMKLR1 and GPR1.
A. Real-time measurement of BRET signal in HEK293T cells expressing either β-arrestin1-RLuc only (✳) or together with CMKLR1-Venus (●), GPR1-Venus (◯) or CCRL2-Venus (△), following stimulation by 100 nM chemerin. B. Real-time measurement of BRET signal in HEK293T cells expressing either β-arrestin2-RLuc only (✳) or together with CMKLR1-Venus (●), GPR1-Venus (◯) or CCRL2-Venus (△), following stimulation by 100 nM chemerin. C-D Localization of β-arrestin in cells coexpressing β-arrestin1-EYFP (C) or β-arrestin2-GFP and CMKLR1 or GPR1, before (NS) and 5 minutes after stimulation with 100 nM chemerin. E-F. Real-time measurement of BRET signal in HEK293T cells expressing β-arrestin1-Rluc and CMKLR1-Venus or GPR1-Venus following stimulation with increasing concentrations of chemerin (●) or the chemerin 9 nonapeptide (◯). G-H Real-time measurement of BRET signal in HEK293T cells expressing β-arrestin2-Rluc and CMKLR1-Venus or GPR1-Venus following stimulation with increasing concentrations of chemerin (●) or the chemerin 9 nonapeptide. Results of BRET experiments are expressed as the difference in BRET signals measured in the presence and absence of chemerin. Data represent the mean ± S.E.M. of three independent experiments.