Signaling Properties of Chemerin Receptors CMKLR1, GPR1 and CCRL2
Fig 4
Determination of the range of G proteins activated by chemerin receptors.
Real-time measurement of BRET signal in HEK293T cells coexpressing G protein biosensors and CMKLR1, GPR1, CCRL2, GPR44, GPR33 or FPR1, following stimulation for 1 minute by 100 nM chemerin. Results are expressed as the difference in BRET signals measured in the presence and absence of chemerin. As controls, cells expressing the α2c adrenergic receptor (Gαi/o) were stimulated with UK14304; cells expressing the AT1 angiotensin receptor (Gα11/q) were stimulated with angiotensin II; cells expressing the β2 adrenergic receptor (Gαs) were stimulated with isoproterenol; cells expressing thromboxane A2 receptor (Gα12/13) were stimulated with U46619. Data represent the mean ± S.E.M. of three to six independent experiments. Statistical significance was assessed using Tukey's test (***P < 0.0001).