Signaling Properties of Chemerin Receptors CMKLR1, GPR1 and CCRL2
Fig 2
A CHO-K1 cells expressing CMKLR1, GPR1 or CCRL2 were incubated with 0.1 nM [125I]-[145–157]-chemerin only (TOTAL) or 0.1 nM [125I]-[145–157]-chemerin in combination with an excess of chemerin or chemerin 9 as competitors. B. CHO-K1 cells expressing CMKLR1 or GPR1 were incubated with 0.1 nM [125I]-[145–157]-chemerin as tracer and increasing concentrations of unlabelled chemerin (●) or chemerin 9 (◯) as competitors. The data were normalized for nonspecific binding (0%) in the presence of 300 nM chemerin, and specific binding in the absence of competitor (100%). The displayed data represent the mean ± S.E.M. of three independent experiments.