Signaling Properties of Chemerin Receptors CMKLR1, GPR1 and CCRL2
Fig 1
A-C. Saturation binding assay using CHO-K1 cells expressing CMKLR1 (A), GPR1 (B) or CCRL2 (C) that were incubated with increasing concentrations of [125I]-chemerin (total binding, ●). Non-specific binding (◯) was determined in the presence of a 100-fold excess of unlabeled chemerin and specific binding (■) was calculated as the difference. D-F. Competition binding assay using CHO-K1 cells expressing CMKLR1 (D), GPR1 (E) or CCRL2 (F) that were incubated with 0.1 nM [125I]-chemerin as tracers and increasing concentrations of unlabelled chemerin (●) or the nonapeptide chemerin 9 (◯) as competitors. The data were normalized for nonspecific binding (0%) in the presence of 300 nM chemerin, and specific binding in the absence of competitor (100%). The displayed data represent the mean ± S.E.M. of three independent experiments.