Long Term Ex Vivo Culture and Live Imaging of Drosophila Larval Imaginal Discs
Fig 5
Proliferation and migration of retinal basal glia in cultured eye disc.
Data were taken from S6 Movie. A mid-L3 repo-RFP.nls eye-antennal disc was cultured and monitored at 25°C for 11.5 hr, Nuclear RFP (green) marks the RBG nuclei. The Repo+ nuclei present at time 0 (A) are artificially marked in blue. The new Repo+ nuclei were marked in white (B). (C, C’) Division in two cells are shown (arrows). The two daughter cells of a division event are linked by a line. The sites of their divisions are marked by blue dots. (D) In repo>Fucci ex vivo cultured eye disc, a cell can undergo a full cell cycle (arrowhead showing progression from red (S phase) to no signal (mitosis phase) to two green (G1 phase) daughter cells). A cell can undergo endoreplication (arrows showing progression from yellow (G2 phase) to green (G1 phase) without undergoing mitosis). (E) The track of each Repo+ nucleus was recorded and color coded, with blue representing the start and red representing the end of movement. The two large Repo+ nuclei are the carpet glia. They do not undergo extensive movement. (F) The migration tracks are presented as displacements from their origin. (F’) The track of a few cells are presented. N = 3. The scale bar is 30 μm.