Generation of VDR Knock-Out Mice via Zygote Injection of CRISPR/Cas9 System
Fig 4
Detection of the CRISPR/Cas9 mediated off-target cleavages in vivo.
(A) PCR products of the potential off-target sites from founder mice. OT1 and OT5 were selected and amplified from genomic DNA of VDR knockout mice. (B) Detection of off-target cleavage by VDRT1 sgRNA at OT1 and OT5 by T7E1 assay. (C) Sequencing results of PCR products.