Generation of VDR Knock-Out Mice via Zygote Injection of CRISPR/Cas9 System
Fig 3
Expression levels of VDR, Cyp27B1 and Cyp24A1 in different tissues from founder mice.
(A) Phenotypes of VDR knock-out mice with different ages. (i,ii,iii) VDR-/- mouse #4 at two, three and four months old, respectively; (iv) VDR-/- mice #26,#9 and VDR-/+ #17 mouse at four months old; (v) wild type mice at four months old. (B) immunofluorescence histochemistry of VDR in mouse small intestine and muscle. Up panel, D-6/DAPI colocalization in the sections of small intestine. Down panel, D-6/DAPI colocalization in the sections of muscle. (C) The differential expression of Cyp27B1 of kidney, skin, small intestine and muscle by qPCR between VDR knockout mice and WT mice. (D) The differential expression of Cyp24A1 in mouse kidney, skin, small intestine and muscle.