Generation of VDR Knock-Out Mice via Zygote Injection of CRISPR/Cas9 System
Fig 2
Detection of CRISPR/Cas9 mediated modifications of VDR in mice.
(A) PCR products of the targeted region of VDRT1 from founder mice. (B) Detection of cleavage of VDRT1 by T7E1 assay. (C) Sequencing results of re-edited VDRT1 loci detected in founder mice.