Spheroid Culture of Head and Neck Cancer Cells Reveals an Important Role of EGFR Signalling in Anchorage Independent Survival
Fig 5
Activation of EGFR protects HNSCC cells against anoikis.
Cells were seeded at a density of 300,000 cells/well in non-coated or poly-HEMA coated 6-well plates and were immediately treated with either EGFR ligands or blocking reagents. After 72h, cells from monolayer (M) and forced suspension (FS) cultures were harvested and the apoptotic fraction was determined by PI staining and subsequent flow cytometry. Bars represent the mean percentages of apoptotic cells ± SD of at least three independent experiments. Significant changes compared to control are marked with an asterisk.