Dietary Flavones as Dual Inhibitors of DNA Methyltransferases and Histone Methyltransferases
Fig 3
Interaction of dietary flavones with AT-rich and GC-rich DNA sequences.
(A) UV-Vis spectra of AT-rich and GC-rich DNA sequences, with 5-aza-2’deoxycytidine, (B) Apigenin, (C) Chrysin, and (D) Luteolin. 100 bp highly GC-rich oligos mimicking CpG islands ranging from -183 to-83 and AT-rich oligos from the -486 to-386 region of the GSTP1 promoter were synthesized and used as a DNA substrate (2.5 mM) for binding with flavones at a 0.5 mM concentration. Absorption spectra of solutions were recorded from 220 nm to 500 nm using Nanodrop. The experiment was repeated three times with similar results. Details are described in the Materials and Methods section.