The Recently Identified Isoleucine Conjugate of cis-12-Oxo-Phytodienoic Acid Is Partially Active in cis-12-Oxo-Phytodienoic Acid-Specific Gene Expression of Arabidopsis thaliana
Fig 3
Relative transcript levels of GRX480 (A) and ZAT10 (B) in seedlings of Arabidopsis thaliana WS, opr3, Col-0 and jar1 after treatment with OPDA or OPDA-Ile.
10-days-old seedlings grown in liquid culture were treated with 30 μM OPDA or 30 μM OPDA-Ile for 30 min (white bars). Two independent controls (black bars) were performed by treatment with bi-distilled H2O containing 0.87% [v/v] and 0.56% [v/v] acetonitrile, respectively. Relative transcript levels were quantified by qRT-PCR using AtPP2A as reference. Each value is represented by the mean of three independent biological replicates ± SD. Treatments and controls were pairwise compared by the Student’s t-test, *p≤0.05, **p≤0.01, ***p≤0.001.