Digital Droplet PCR for the Absolute Quantification of Exon Skipping Induced by Antisense Oligonucleotides in (Pre-)Clinical Development for Duchenne Muscular Dystrophy
Fig 6
Comparison of different PCR methods on patient-derived muscle cell cultures.
Quantification of exon 51 skip levels in patient-derived muscle cell cultures (Δ48–50), transfected with an exon 51 skipping AON (400nM) by (A) ddPCR analysis, (B) qPCR analysis and (C) conventional primary PCR (20/30/40 cycles) and nested PCR (44 cycles). (D) Low background levels of exon 51 skipping were detected by ddPCR and qPCR in the non-treated (NT) samples. N/a indicates that no exon skip percentage could be calculated. Percentages shown are mean (n = 3) with SEM.