Tension Recovery following Ramp-Shaped Release in High-Ca and Low-Ca Rigor Muscle Fibers: Evidence for the Dynamic State of AMADP Myosin Heads in the Absence of ATP
Fig 7
Tension response of skinned fibers in low-Ca rigor state.
(A) Tension recovery following repeated releases (amplitude, 0.5% of Lo; duration, 5ms). Note that tension recovery takes place from negative tension below zero tension baseline. (B) Disappearance of tension recovery following repeated releases (amplitude, 0.5% of Lo; duration, 5ms) in the presence of 10mM EDTA. Note that the tension drop coincident with applied release is not followed by tension recovery. (C) Disappearance of tension recovery following repeated releases (amplitude, 0.3% of Lo; duration, 5ms) in the presence of 10mM EDTA. In A, B and C, downward arrow indicates time of application of Low-Ca rigor solution (pCa, > 9), while horizontal broken line indicates zero tension baseline.