The Use of Poly-L-Lysine as a Capture Agent to Enhance the Detection of Antinuclear Antibodies by ELISA
Fig 6
Titration of plasmas against STS-supernatant captured with PLL or coated directly in ELISA plate wells.
Index plasmas and normal plasmas were titrated against 1 μg/ml STS-supernatant coated directly in ELISA plate wells or captured onto wells pre-coated with 500 ng/ml PLL. Plasma dilutions ranged from 1/800 to 1/25,600; buffer only was used as control. Squares show data for STS-supernatant directly coated on plate; circles show data for STS-supernatant captured by polymer. Index plasmas used contained the specificities indicated on the charts in the figure.