The Use of Poly-L-Lysine as a Capture Agent to Enhance the Detection of Antinuclear Antibodies by ELISA
Fig 5
The effect of RNase A treatment of apoptotic supernatant on binding in the ELISA.
STS-supernatant was prepared as described and treated with a range of RNase A concentrations from 1.5 Kunitz units/ml to 0.015 Kunitz units/ml or with buffer alone. Samples that were digested and controls were assayed in both a direct coat ELISA and a PLL capture ELISA. SLE plasma 1 was used at 1/800 dilution. Squares show data for PLL capture; circles show data for direct coat ELISA. Data represent 1 well per condition.