Practical Recommendations for the Use of the GeneSwitch Gal4 System to Knock-Down Genes in Drosophila melanogaster
Fig 5
Expression of RNAi transgenes does not correlate with the presence of RU-486 in the fly food.
(A) Western blot analysis of ND-39, NDI1 and ATP5A levels in control, induced and non-induced groups. (B) Quantification of A (n = 2). (C) Respirometry in tubGS flies with and without an ND-39 RNAi transgene (n = 3–8). (D) Western blot analysis of ND-39 and NDI1 levels in control, induced and non-induced groups. (E) Quantification of D (n = 2–3). (F) Western blot analysis of ND-39 levels in controls and experimental flies fed with RU-486 during development (1 μM), development and adulthood (1 μM and 500 μM respectively) or exclusively during adulthood (500 μM) (n = 2–3). All flies were 5 days old when proteins were extracted. Flies that were exclusively fed during development spent 5 days in food without RU-486. GAPDH is shown as loading control. daGS, daughterless-GeneSwitch GAL4; tubGS, tubulin-GeneSwitch GAL4; +/- indicate presence/absence of the transgene or 500 μM RU-486 (inducer).