SOFI Simulation Tool: A Software Package for Simulating and Testing Super-Resolution Optical Fluctuation Imaging
Fig 5
High-labelling density simulations.
Widefield, SOFI and FALCON STORM images of the simulated structures labelled with a relatively high density of emitters (1000/μm2) Standard conditions represent a scenario well suited for STORM. in the case of Short acquisition time, the super-resolution images are generated from an image sequence of only 1000 frames. Short acquisition time and higher on-time ratio represent a situation with very high density of activated emitters per frame which makes it challenging for STORM algorithms. Emitters shown in the left column are enlarged for the visualization purposes. All parameters of the standard conditions can be found in the S1 Appendix and on our project website [16].