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Novel Flow Cytometry Analyses of Boar Sperm Viability: Can the Addition of Whole Sperm-Rich Fraction Seminal Plasma to Frozen-Thawed Boar Sperm Affect It?

Fig 6

Effects of treatment (A) and time (B) on plasma and acrosomal membrane integrity and mitochondrial membrane potential. CT—control; CS—centrifuged and suspended in autologous seminal plasma (SP); CW—centrifuged and withdrawn SP; CWSP—CW containing autologous seminal plasma. IPIAH—plasma and acrosome membrane integrity and high Δψm. Different letters represent a significant difference (p < 0.05).

Fig 6

doi: https://doi.org/10.1371/journal.pone.0160988.g006