A Single-Batch Fermentation System to Simulate Human Colonic Microbiota for High-Throughput Evaluation of Prebiotics
Table 1
Changes in production of acetate, propionate, and butyrate after 24 h of fermentation in the single-batch systems using medium supplemented with the prebiotics (FOS, GOS, IMO, XOS, raffinose, lactulose, or lactosucrose).
Samples of human feces (designated F37, F23, M38, F35, M24, or M43) were used to inoculate each system. Changes are presented as the ratio of concentration in the experimental system normalized to that in the control system (without added prebiotics). The control system generated acetate, propionate, and butyrate at concentrations (mean±SD, n = 6) of 104.0±16.3, 28.4±10.1, and 14.8±5.3 mM, respectively.