A Single-Batch Fermentation System to Simulate Human Colonic Microbiota for High-Throughput Evaluation of Prebiotics
Fig 1
Phylum-level compositional view of bacteria in three human fecal samples/inocula (–) (F37-1: female, age 37; F37-2: female, age 37; and M54: male, age 54) and corresponding cultures at 6, 9, and 24 h after the initiation of fermentation.
A compositional view of bacterial phyla based on the taxonomic assignment of 16S rRNA genes is shown. Bacterial composition of each sample was estimated from the results of the RDP classifier. Eubacterial 16S rRNA gene copy numbers are shown in the right column; units are copies/wet-g-feces (inoculum) or copies/mL-culture (fermentation).