Engineered Production of Short Chain Fatty Acid in Escherichia coli Using Fatty Acid Synthesis Pathway
Fig 1
Schematic representation of various strategies adopted to engineer E. coli for production of butyric acid.
Fatty acid synthesis and degradation cycles have been depicted with blue and orange arrows, respectively. Regulatory proteins have been shown in dotted box in green and red color denoting activation and repression, respectively. Genes that have been overexpressed in this study have been denoted with green upward arrow, while genes that have been deleted are shown along with red cross. Abbreviations: TesAT—thioesterase of Anaerococcus tetradius; TesBT–thioesterase of Bacteroides thetaiotaomicron; TesBF—thioesterase of Bryantella formatexigens; Acc*—acetyl-CoA carboxylase of Corynebacterium glutamicum; FabD–malonyl CoA-ACP transacylase; FabH - β- ketoacyl-ACP synthase III; FabG - β-ketoacyl-ACP reductase; FabA and FabZ - β-hydroxyacyl-ACP dehydratase; FabI—enoyl-ACP reductase; FabB - β-ketoacyl-ACP synthase I; FadD—fatty acyl-CoA synthetase; FadE—acyl CoA dehydrogenase; FabR–transcriptional repressor of FASII; FadR–transcriptional enhancer of FASII and repressor of β-oxidation.