Allostimulatory Effects of Dendritic Cells with Characteristic Features of a Regulatory Phenotype
Fig 2
LiClPAM3 dendritic cells (DCs) are potent T cell stimulators and induce Th1 and Th17 differentiation in vitro.
The T cell stimulatory capacity of (C57Bl/6N) LiClPAM3 DCs was tested in mixed lymphocyte reaction. (Balb/c) T cell proliferation was analyzed by dilution of CFSE signal (A). LiClPAM3 DC stimulation resulted in higher T cell proliferation at days 3, 4, 5, and 6 compared to control DC stimulation (*p<0.05) (B). T cell differentiation was analyzed by measuring cytokine in co-culture supernatants in ELISA. Interferon-γ (C), but not IL-17 (D) production, by LiClPAM3 DC stimulated T cells was higher compared to LPS DC stimulated T cells at day 4 (* p<0.05). IL-4, IL-6 and IL-23 levels did not differ between groups (E). Results are of 3 independent experiments.