Virtual Hematoxylin and Eosin Transillumination Microscopy Using Epi-Fluorescence Imaging
Fig 5
Virtual transillumination H&E images rendered from epi-fluorescence MPM imaging.
a) Virtual transillumination Beer’s law algorithm. b) An additive method with nonlinear transfer function. c) Additive method with linear transfer function. The virtual transillumination algorithm enables both high contrast, realistic color rendering and perfect white point. In contrast, the additive method with nonlinear transfer function has reduced color accuracy due to the nonlinear transfer function shifting color slightly and minor green-shifting the backlight color to RGB [0.9 1.0 0.9], while the additive linear method has good color accuracy and perfect white point, but lower contrast. Of note, all three images span the entire 0.0 to 1.0 contrast range, and additional contrast enhancement is not possible without applying a nonlinear transfer function.