Novel Bioluminescent Binding Assays for Ligand–Receptor Interaction Studies of the Fibroblast Growth Factor Family
Fig 1
(A) Schematic presentation of the NanoLuc-conjugated FGF2. The structure of [C95S]FGF2 was adopted from the previously published FGF2 crystal structure (PBD ID: 2BFH) [22]. Positions of the Cys residues, Y32, and Y111 in FGF2 are labeled and their side-chains are shown. (B) The previously solved crystal of structure of FGF2 in complex with receptor FGFR1 and heparin (PBD ID: 1FQ9) [24]. FGF2 molecules are shown in red and the positions of their Cys residues, Y32, and Y111 are labeled.