The Anti-Inflammatory Effects and Mechanisms of Eupafolin in Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages
Fig 4
The eupafolin-mediated inhibition of the LPS-induced NO, iNOS, COX-2 and PGE2 expression in RAW264.7 macrophages involved MAPK activation.
The RAW264.7 macrophages were treated for 1 h with 30 μM of the MAPK inhibitors or 10 μM of the PI3K/AKT inhibitor and were then incubated with 1 μg/ml of LPS for 24 h. (A) COX-2 and (B) iNOS protein expression was determined by Western blot analysis. β-actin was processed in parallel as an internal control for protein loading. (C) NO was measured with the Griess assay, and (D) PGE2 was measured with an ELISA assay. The data are shown as the means ± SEM (n = 5–8). *P<0.05 vs. the untreated control, †P<0.05 vs. the LPS-treated cells. ‡P<0.05 vs. the LPS+EUP treated cells.