The Anti-Inflammatory Effects and Mechanisms of Eupafolin in Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages
Fig 3
Eupafolin inhibited the LPS-induced activation of MAPKs in RAW264.7 macrophages.
The RAW264.7 macrophages were pretreated with 1 μg/ml of LPS for various times as indicated. The phosphorylated and total (A) ERK, (B) JNK, (C) p38, or (D) AKT levels were determined by Western blot analysis. Total ERK (t-ERK), total JNK (t-JNK), total p38 (t-p38), or total AKT (t-AKT) protein was used as the loading control. The data are shown as the means ± SEM (n = 6). *P<0.05 vs. the untreated control. The cells were treated for 1 h with 60 μM eupafolin and were then incubated with 1 μg/ml of LPS for 30 min. The phosphorylated (E) ERK, (F) JNK, (G) p38, or (H) AKT levels were determined by Western blot analysis. Total ERK (t-ERK), total JNK (t-JNK), total p38 (t-p38), or total AKT (t-AKT) protein was used as the loading control. The data are shown as the means ± SEM (n = 6). *P<0.05 vs. the untreated control, †P<0.05 vs. the LPS-treated cells.