Mutation in the Pro-Peptide Region of a Cysteine Protease Leads to Altered Activity and Specificity—A Structural and Biochemical Approach
Fig 2
SDS-PAGE analyses of the proteases.
a) Purified pro-proteases. b) Gelatin gel zymography for WT (TS) and three mutants. 2μg pro-proteases were loaded in each lane. The lanes of each gel are marked at the bottom. M lane is for molecular weight marker whose values in kDa are given at the left panel.